NAGE · Niagen Bioscience, Inc.

Shares acquired pursuant to the Issuer's Employee Stock Purchase Plan. The acquisition is exempt under Rule 16b-3(c) and 16b-3(d).

Shares acquired pursuant to the Issuer's Employee Stock Purchase Plan. The acquisition is exempt under Rule 16b-3(c) and 16b-3(d).

Shares acquired pursuant to the Issuer's Employee Stock Purchase Plan. The acquisition is exempt under Rule 16b-3(c) and 16b-3(d).

Option vests 100% on June 24, 2027.

Option vests 100% on June 24, 2027.

Option vests 100% on June 24, 2027.

Option vests 100% on June 24, 2027.

Option vests 100% on June 24, 2027.

Option vests 100% on June 24, 2027.

Option vests 100% on June 24, 2027.

The Reporting Person exercised the stock options reported on this Form 4 in advance of their expiration on November 15, 2026, paying the exercise price with cash, and has not sold any of the shares received upon exercise.

One-fourth of the shares subject to the option shall vest on each anniversary of the grant date.

One-fourth of the shares subject to the option shall vest on each anniversary of the grant date.

One-fourth of the shares subject to the option shall vest on each anniversary of the grant date.

The weighted average purchase price for the transaction reported was $6.81, and the range of prices were between $6.7975 and $6.82, inclusive. Upon request by the SEC staff, the issuer, or any security holder of the issuer, full information regarding the number of shares purchased at each separate price within the range above will be provided.

Option vests 100% on June 24, 2026.

Option vests 100% on June 24, 2026.

Option vests 100% on June 24, 2026.

Option vests 100% on June 24, 2026.

Option vests 100% on June 24, 2026.

Option vests 100% on June 24, 2026.

Option vests 100% on June 24, 2026.

The Reporting Person exercised the stock options reported on this Form 4 in advance of their expiration on July 29, 2025, paying the exercise price with cash, and has not sold any of the shares received upon exercise.

Each Performance Stock Unit ("PSU") represents a contingent right to receive one share of the company's common stock when vested. The PSUs vest incrementally upon the Company's common stock achieving specified price per share performance goals and continued employment prior to February 25, 2032, and subject to a change in control.

One-third of the shares subject to the option shall vest on February 12, 2026 and the remaining shares subject to the option shall vest in a series of 24 equal monthly installments thereafter.

One-third of the shares subject to the option shall vest on February 12, 2026 and the remaining shares subject to the option shall vest in a series of 24 equal monthly installments thereafter.

One-third of the shares subject to the option shall vest on February 12, 2026 and the remaining shares subject to the option shall vest in a series of 24 equal monthly installments thereafter.

The weighted average sale price for the transaction reported was $6.1881, and the range of prices were between $6.13 and $6.285. Upon request by the SEC staff, the issuer, or any security holder of the issuer, full information regarding the number of shares sold at each separate price will be provided.

The sale of shares was solely to cover the exercise cost and associated taxes for the exercise of the options which were scheduled to expire on July 5, 2025. As a result of the exercise of the options, the Reporting Person will own 12,840 more shares.

The weighted average purchase price for the transaction reported was $7.36, and the range of prices were between $7.345 and $7.36, inclusive. Upon request by the SEC staff, the issuer, or any security holder of the issuer, full information regarding the number of shares purchased at each separate price within the range above will be provided.

One-third of the shares subject to the option shall vest on October 21, 2025 and the remaining shares subject to the option shall vest in a series of 24 equal monthly installments thereafter.

The weighted average sale price for the transaction reported was $3.57, and the range of prices were between $3.52 and $3.64. Upon request by the SEC staff, the issuer, or any security holder of the issuer, full information regarding the number of shares sold at each separate price will be provided.

On August 20, 2024, Brilliant Dynasty Limited, a British Virgin Islands corporation ("Brilliant Dynasty") entered into a securities purchase agreement with Prime Tech Global Limited, a British Virgin Islands corporation ("Prime Tech") and Alpha Mount International Limited, a British Virgin Islands corporation ("Alpha") to purchase all of the share capital of Champion River Ventures Limited, a British Virgin Islands corporation ("Champion River"), Winsave Resources Limited, a British Virgin Islands corporation ("Winsave"), and Radiant Treasure Limited, a British Virgin Islands corporation ("Radiant") from Prime Tech and Alpha, for an aggregate consideration of $32,058,489.15 (the "August 2024 Transaction"). At time of entry into the securities purchase agreement, Champion River, Winsave and Radiant beneficially owned 7,940,937, 3,088,433 and 379,345 shares of common stock of the Issuer, respectively.

Of the total shares beneficially owned following the reported transaction (i) 6,917,783 shares of the Issuer's common stock are directly beneficially owned by Pioneer Step Holdings Limited, a British Virgin Islands corporation ("Pioneer Step"), (ii) 420,000 shares of the Issuer's common stock are directly beneficially owned by Dvorak International Limited ("Dvorak International"), (iii) 547,858 shares of the Issuer's common stock are directly beneficially owned by Skyinvest Associates Limited ("Skyinvest Associates"), (iv) 7,940,937 shares of the Issuer's common stock are directly beneficially owned by Champion River, (v) 3,088,433 shares of the Issuer's common stock are directly beneficially owned by Winsave, and (vi) 379,345 shares of the Issuer's common stock are directly beneficially owned by Radiant.

(continued from Footnote 2) Elgar International Limited, a British Virgin Islands corporation ("Elgar International") is the sole shareholder of Brilliant Dynasty. Brilliant Dynasty will be the sole shareholder of Champion River, Winsave and Radiant upon the completion of the August 2024 Transaction. As such, each of Elger International and Brilliant Dynasty may be deemed to beneficially own the shares of the Issuer's common stock directly held by Champion River, Winsave and Radiant. Chau Hoi Shuen Solina Holly ("Solina Chau") is the sole shareholder of each of Pioneer Step, Dvorak International, Skyinvest Associates and Elgar International. As such, Solina Chau may be deemed to beneficially own the shares of the Issuer's common stock directly held by Pioneer Step, Dvorak International, Skyinvest Associates, Champion River, Winsave and Radiant.

On August 20, 2024, Prime Tech Global Limited, a British Virgin Islands corporation ("Prime Tech") and Alpha Mount International Limited, a British Virgin Islands corporation ("Alpha") entered into a securities purchase agreement with Brilliant Dynasty Limited, a British Virgin Islands corporation ("Brilliant Dynasty"), to sell all of the share capital of Champion River Ventures Limited, a British Virgin Islands corporation ("Champion River"), Winsave Resources Limited, a British Virgin Islands corporation ("Winsave") and Radiant Treasure Limited, a British Virgin Islands corporation ("Radiant") to Brilliant Dynasty, for an aggregate consideration of $32,058,489.15. At time of entry into the securities purchase agreement, Champion River, Winsave and Radiant beneficially owned 7,940,937, 3,088,433 and 379,345 shares of common stock of the Issuer, respectively.

The sole shareholder of Prime Tech is Mayspin Management Limited ("Mayspin"), and the sole shareholder of Mayspin is Li Ka Shing. The sole shareholder of Alpha is Apex Dynasty Limited, a British Virgin Islands corporation ("Apex"), and the sole shareholder of Apex is Li Ka Shing.

Option vests 100% on June 20, 2025.

Option vests 100% on June 20, 2025.

Option vests 100% on June 20, 2025.

Option vests 100% on June 20, 2025.

Option vests 100% on June 20, 2025.

Option vests 100% on June 20, 2025.